【摘 要】
：
NB-ARC domain proteins are one of the most diverse resistant proteins (R) in plant,which play a crucial role in effector-triggered immunity (ETI).Previous reports have indicated that some NB-ARC domai
【机 构】
：
Institute of Chinese Crude Drugs GAP, Fujian Agriculture and Forestry University, Fuzhou, China, 350
【出 处】
：
2016首届中国中药资源大会暨CSNR中药及天然药物资源研究专业委员会第十二届学术年会
论文部分内容阅读
NB-ARC domain proteins are one of the most diverse resistant proteins (R) in plant,which play a crucial role in effector-triggered immunity (ETI).Previous reports have indicated that some NB-ARC domain proteins were involved in responding processes of R.glutinosa consecutive monoculture practice.However, NB-ARC family proteins of R.glutinosa are little known to date.In this study, 45 NB-ARC proteins were identified from R.glutinosa protein library using hide markov model (HMM) and bioinformatics methods.Homology analysis showed that these proteins have highly homologous with Tubiflorae plants including Pedaliaceae, Scrophularuaceae, Solanaceae, Asteraceae, etc.The most of them have a typical characteristic with combining coiled-coiled domain type.A highly conserved ATP bind domain with 288 aa length containing 12 α-helix and 4 β-strand and barrel structure core of subunit enclosed by 4 parallel β-strands were observed to present in most of NB-ARC proteins by protein structure prediction.Additionally, we also found that NB-ARC domain proteins in R.glutinosa had strong homology to the R1A and RGA proteins, which participated to reorganization of Avrl effectors and salicylic acid-mediated signal transduction.Most of the closely related members in the phylogenetic tree exhibited common motifs as expected.The expression patterns analysis of 36 NB-ARC family proteins indicated that the effective immune resistances were triggered approximately at 30 days after planting in R.glutinosa.Then it might be more sensitive period to induce occurrence of consecutive monoculture problems at the next 30 days.
其他文献
目的：应用原子吸收光谱法,测量窝儿七和其生长土壤中的7种金属元素含量,分析二者中金属含量的相关性,研究金属元素对窝儿七根茎生长的影响。方法：原子吸收光谱法。结果：窝儿七的Cu,Ca,Mg,Zn,Mn,Fe与土壤中的同类元素成正相关性,Pb含量与土壤无关。结论：窝儿七中7种金属元素与土壤有密切关系。
氮杂苯基非那烯酮是一类仅分布于血皮草科植物的生物碱。本研究发现该类生物碱主要分布于血皮草科植物Xiphidium caeruleum花的雄蕊中，并从中分离鉴定氮杂苯基非那烯酮类化合物19个。运用稳定同位素13C标记和组织培养技术，构建了该植物中苯基非那烯酮的生物合成及代谢网络。在此基础上，利用植物材料和伯胺类物质一锅合成氮杂苯基非那烯酮23个，部分化合物显示了中等程度的抑制肿瘤细胞生长作用。氮杂苯
生态化学计量学是近年来新兴的一个生态学研究领域，是研究生物系统能量平衡和多重化学元素平衡的科学，是元素平衡对生态交互作用影响的一种理论，主要研究生态过程中化学元素的比例关系。探讨碳、氮、磷生态化学计量学特征，可以更好地揭示生态系统内植物—土壤养分元素相互关系。本文综述了生态化学计量学在植物营养以及在药用植物资源研究的现状。并对药用植物的生态化学计量学研究方向进行了展望，提出生态化学计量学在药用植物
目的：了解中药材农药残留限量标准及污染现状.方法：查阅欧盟、英国、美国、韩国、日本和中国药典中对农药残留的限量标准，进行比较分析；以相关文献提供的中药材农药含量数据为基础，使用SPSS 20.0软件进行统计分析；并以《欧洲药典》规定的农药残留最大限量为标准，判断中药材农药残留超标情况.结果：各国药典对农药残留的规定项目和限量标准存在差异；中药材中存在不同程度的农药残留污染，超标率较高的农药有虫螨畏
针对药用植物施肥研究整体水平较低的现状，对山东、河北、山西、内蒙、宁夏、甘肃6省进行中药材施肥情况走访调查，调查发现中药材施肥沿用传统农作物方式，存在盲目滥用不合理施肥现象。总结中国北方地区中药材施肥现状，为中药材规范化施肥提供一定的参考，并提出了今后中药材施肥发展的一些建议。
The practice of consecutive monoculture results in complex environment stress mediated by multiple factors.The main inducing factors of the problems associated with consecutive monoculture are autotox
Preliminary studies have widely investigated the dose-effect relation of "plant-soil-micro" system for R.glutinosa, a traditional Chinese herb with severe consecutive monoculture problems, based on di
Rehmannia glutinosa Libosch (R.glutinosa), a perennial herbaceous medicinal plant,belongs to the Scrophulariaceae family.R.glutinosa is rich in medicinal value and has a long history of cultivation.Ho
目的：考察凉山州栽培益母草的最佳播种期和采收期，为当地益母草的生产提供相应的科学依据.方法：分别考察春播益母草和秋播益母草不同播种期和采收期药材的产量和有效成分含量变化情况，产量以每小区产益母草药材干重记，有效成分含量按照《中国药典》2015年版一部益母草项下规定方法对盐酸水苏碱和盐酸益母草碱进行测定.结果：不同播种期益母草药材产量及有效成分含量变化均呈单峰曲线，春播益母草在3月18日达到最高，秋
Consecutive monoculture problems significantly suppress the yield and quality of Rehmannia glutinosa Libosch.Previous studies have shown that the spent substrate of Pleurotus eryngii Quel positively a