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In the present study,the possible involvement of reactive oxygen species(ROS)in prothoracicotropic hormone(PTTH)-stimulated ecdysteroidogenesis of Bombyx mori prothoracic glands(PGs)was investigated.PTTH treatment resulted in a rapidly transient increase in the intracellular ROS concentration,as measured using 2,7-dichlorofluorescin diacetate(DCFDA),an oxidation-sensitive fluorescent probe.