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Background:To investigate the relationship between HBV DNA BCP/PC region quasispecies features with relapse until meeting endpoint criteria in CHB patients with LAM treatment Methods:43 CHB patients liver biopsy samples were assessed who had met the endpoint criteria of 2008 APASL,including 2 groups:25 patients with LAM-naive treatment,18 with NAs retreatment who had received LAM-naive treatment.Serum were collected at baseline of treatment and relapse.HBV rcDNA and cccDNA were cloned into plasmid and transformed into competent cells.Selected 20 to 32 of positive clones for sequencing randomly.