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Objective: To explore insulin signal change in HepG2 cell exposed to IHand explore potential role of ERS under this.Method: 1.cell was divided: room air 8h(RA8h), RA16h, RA24h, IH 8h, IH16h, IH24h.Oxy
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Objective: To explore insulin signal change in HepG2 cell exposed to IHand explore potential role of ERS under this.Method: 1.cell was divided: room air 8h(RA8h), RA16h, RA24h, IH 8h, IH16h, IH24h.Oxygen in IH wasdynamically from 21％ to 1％, and remained 21％ in RA.Insulin was input, p-AKT and AKT was measured.2.JNK and ERS proteins were measured after IH or RA.3.PBA preincubation for 8h, cell was exposed to RA or IH for 24h, ERS markers were examined.p-AKTexamined in other groups with insulin
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