论文部分内容阅读
Objective: With the potential uses of photothermal therapy (PTT) in cancer treatment for the amazing efficacy, and the growing concerns of nanotoxicity of the hyperthermia agents such as carbon nanotubes and gold-based nanomaterials, the importance of searching a biocompatible hyperthermia agent cannot be emphasized too much.In this work, a novel promising hyperthermia agent employing magnetic Fe304 particles with fairly low toxicity wasinvestigated.Methods: The magnetic nanoparticles were sythesized using a modified solvothermal reaction and then modified with O-Carboxymethyl chitosan (CMCTS).Then the photothermal effect, photothermal toxicity, biodistribution and cancer therapeutic effect of the magnetic nanoparticles were evaluated.Results: After modification with carboxymethyl chitosan (CMCTS), the obtained Fe304@CMCTS particles could disperse stably in PBS and serum without any aggregation.In cytotoxicity experiment, Fe304@CMCTS particles exhibited excellent photothermal effect and fairly low cytotoxicity.The in vivo fluorescence images and magnetic resonance (MR) images of mice revealed that by attaching a magnet to the tumor, Fe304@CMCTS particles accumulated in tumor after intravenous injection and showed low distribution in liver.After being exposed to 808 nm laser for 5 min at a low power density of 1.5 W·cm-2, the tumors on Fe304@CMCTS-injected mice reached a temperature of~52 ℃ and were completely destructed.Conclusions: A novel multifuncional magnetic nanoparticles with extremely low toxicity and simple structure for simultaneous MR imaging, targeting drug delivery and photothermal therapy can be facilely fabricated.Our work suggests that the constructed magnetic nanoparticles has promising potential in PTT of cancer.