【摘 要】
：
Microvitellogenin has been characterized only from lepidopteran species M.sexta.In this paper,a novel microvitellogenin was isolated and characterized from Antheraea pernyi (Lepidoptera: Saturniidae).
【机 构】
：
辽宁省昆虫资源工程技术研究中心,生物科学与技术学院,沈阳农业大学,沈阳 110866 辽宁省野蚕研
【出 处】
：
第十届家(柞)蚕遗传育种及良种繁育学术研讨会
论文部分内容阅读
Microvitellogenin has been characterized only from lepidopteran species M.sexta.In this paper,a novel microvitellogenin was isolated and characterized from Antheraea pernyi (Lepidoptera: Saturniidae).The resulting 928 bp cDNA sequence contains an open reading frame of 783 bp encoding a polypeptide of 260 amino acids,with a predicted molecular weight of 29.96 kDa.The deduced protein sequence shared the putative conserved domains with lepidopteran low molecular weight lipoprotein belonging to the lipoprotein_11 superfamily.In search of database available,the homologues were found only from lepidopteran insects,including microvitellogenin from M.sexta with a 48% sequence identity,low molecular weight (30 kD) lipoproteins from B.mori with 40–47% sequence identities,and an uncharacterized protein from A.assama with a 64% sequence identity.RT-PCR analyses showed that the mRNA of this gene was specifically expressed in fat body,spermaries and ovaries during pupal stage.Western bloting analysis revealed that the product of this gene was present in the fat bodies and hemolymph of female pupae,and eggs.These results indicated that the target protein is a novel microvitellogenin.RNAi analysis is conducting to probe into the function of this gene.
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