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As one of the most ancient crops, the grape was used by mankind 8,000 years ago in Mesopotamia.Most grapes belong to Vitis vinifera, which include two sub-species: cultivated subspecies (V.vinifera ssp.sativa or ssp.vinifera) and the wild subspecies (V.vinifera ssp.sylvestris).Cultivated subspecies are widely used in agricultural production and planted in the temperate, boreal and tropical regions, while the wild subspecies are primarily used for grape vine resistance breeding stock and raw materials.Paclobutrazol and Piperonyl butoxide (PBO) residues of grapes sprayed with differing concentrations of pesticide were detected by HPLC in this study.Firstly, paclobutrazol and PBO were extracted from samples with 80% methanol under vacuum distillation conditions and then extracted with dichloromethane, rotary evaporation to dryness and dissolved in methanol after the last machine.After high performance liquid chromatography on the standard sample and sample solution, the residue contents of paclobutrazol and PBO were finally determined.The results showed that residue concentrations of paclobutrazol and PBO were not detected after spraying at different times and concentration except in sample 6.Therefore, it is suggested that, in the given range, spraying external hormones to improve production and quality of grapes do not have negative effects on grape fruit safety.