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RNA in spermatozoa was the topic of some heated debate for a long time, but with the advent of new techniques in fields of molecular andrology, more and more evidences have been accumulating suggesting that human ejaculated spermatozoa convincingly retain a complex population of RNAs, those RNAs might play important roles in spermatozoa development, motility and capacitation and even the zygote development.Sperm RNA can be used as a measure of sperm quality and predicting sperm fertilizing capacity.They also played an important role on the research of male infertility and assisted reproductive technology.