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Objective Roles of hippocampal AMPARs were investigated in irritable bowel syndrome like rats (IBS-like rats) to clarify the potential mechanisms underlying the central sensitization in chronic functional visceral hypersensitivity, which may provide a new point for the clinical prevention and pharmaceutical treatment.Methods IBS-like rats were generated by neonatal maternal separation (NMS) for 3 h daily during post-natal days 3-21.