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Miscanthus straw is emerging as a viable feedstock for fuel ethanol production and renewable resources.In this report we have studied the effect of 60Co-γ ray irradiation pretreatment on biochemical composition and enzymatic hydrolysis of miscanthus straw.Compared with the control sample,the cellulose,hemicellulose and lignin of miscanthus straw were greatly degraded by 60Co-γ ray irradiation pretreatment at the doses loading from 400~2000 kGy,their degradation rates were up to 65.9%,78.0%and 14.8%respectively with irradiation pretreatment at the dose of 2000 kGy.