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A eukaryotic expression plasmid encoding carboxyl terminal-truncated HCV envelope protein 2 (E2) of H77 strain was constructed and transfected into human 293T cells.Both intracellular E2 and secreted E2 protein in supernatant could be detected by western blot analysis with anti-E2 McAb.The supernatant was used to assay anti-E2 antibodies in sera of hepatitis C patients by ELISA and 49 of 60 sera samples was detected anti-E2 IgG positive.The full-length envelope protein expression plasmid was transfected into 293 T cells and the reactivity of transfectant with anti-E2 IgG positive sera were analyzed by immunofluorescence.