Co-expression of GR79 EPSPS and GAT yields herbicide-resistance with low-glyphosate-residue cotton

• 作 者: Chengzhen Liang Bao Sun Zhigang Meng Zhaohong Meng Yuan Wang Guoqing Sun Tao Zhu Wei Lu Wei Zhang Waqas Malik Min Lin Rui Zhang Sandui Guo
• 机 构: BiotechnologyResearchInstitute,ChineseAcademyofAgriculturalSciences,Beijing100081,China
• 出 处: 全国农业生物化学与分子生物学第十五届学术研讨会
• 发表日期: 2016年9期
• 关键词: glyphosate GMO GR79 EPSPS GAT cotton

　　Glyphosate resistant (GR) crops have been adopted on a massive scale by North and South Americans.Currently, about 80% of the 120 million hectares ofthe global genetically-modified (GM) crops are GR.However, the adoption of GR plants in China has not occurred at the same pace, owing to several factors specially the residual effects of glyphosate in transgenic plants.Here, we reported the co-expression of codon-optimized forms of GR79 EPSPS and N-acetyltransferase (GAT) genes in cotton.We found five-times more resistance to glyphosate with 10-fold reduction in glyphosate residues in pGR79 EPSPS-pGAT co-expression cottons GGCO2 and GGCO5.Field trials showed that pGR79-pGAT transgenic cotton lines have the similar performance to conventional varieties and also saved USD 390-495 per hectare because of the high cost in conventional weed management practices.Our strategy to pyramid these genes has practical repercussions for the engineering and breeding of high-resistant low-glyphosate-residue cotton.