Antibacterial Action of Selenium-Enriched Probiotics against Pathogenic Escherichia Coli

• 作 者: Jiajun Yang Kehe Huang Shunyi Qin Xianshi Wu Zhiping Zhao Fu Chen
• 机 构: Institute of Nutritional and Metabolic Disorder,Nanjing Agricultural University,Nanjing 210095,China
• 出 处: 中国畜牧兽医学会2008年学术年会暨第一届中国兽医临床大会
• 发表日期: 2008年3期
• 关键词: 富硒益生菌 致病性大肠杆菌 抗菌作用

The purpose of this study was to evaluate the inhibitory activity of selenium-enriched probiotics against pathogenic Escherichia Coli(E.Coli)in vitro and in vivo.E.Coli was co-cultured in vitro with each probiotic strain individually and a mixture of the four strains and its population was counted at various time points.We also collected a cell-free culture supernatant(CFCS)of each probiotic strain and the four-strain mixed to examine their antibacterial activity using the cylinder plate method.Results demonstrated that co-culture with probiotics significantly reduced the number of E.Coli.The different sizes of the inhibition zones made by each CFCS proved that E.Coli was inhibited by the metabolites of the probiotics.In vivo，Kunming mice were allocated into different groups supplemented with selenium-enriched and other probiotics.After 28 days，the mice were inoculated with pathogenic E.Coli to Compare mortality and inspect other indexes of each treatment.The mortality of the group with selenium-enriched probiotics was the lowest.In addition，the organic antioxidant status improved，immunity was fortified，and the intemal environment of the intestinal tract was enhanced with selenium-enriched probiotic supplementation.In Conclusion,selenium-enriched probiotics can strongly antagonize pathogenic E.Coli in vitro and vivo.