论文部分内容阅读
Precise rotation of suspended cells is one of many fundamental manipulations used in a wide range of biotechnological applications such as cell injection and enucleation in nuclear transfer (NT) cloning.Here we present an alternating current (ac) induced electric field-based biochip platform which has an open-top sub-mm square chamber enclosed by four sidewall electrodes and two bottom electrodes to achieve rotation about two axes,thus 3D cell rotation for the first time.Through experiments,we demonstrate rotation of bovine oocytes of ~120 μm in diameter about two axes,with the capability of controlling the rotation direction and rate for each axis through control of the ac potential amplitude,frequency,and phase shift,and cell medium conductivity.The maximum observed rotation rate reached nearly 140°/s,while a consistent rotation rate reached up to 40°/s.This simple,transparent,cheap-to-manufacture,and open-top platform,allows further function modules to be integrated to become a more powerful cell manipulation system.