【摘 要】
：
Aim To investigate whether Kv7 channels opener retigabine could alleviate memory impairment induced by chronic restraint stress (CRS) and the underlying neuroprotective mechanisms.Methods Adult male K
【机 构】
：
Dept of Pharmacology,School of Basic Medicine,Tongji Medical College,Huazhong University of Science
论文部分内容阅读
Aim To investigate whether Kv7 channels opener retigabine could alleviate memory impairment induced by chronic restraint stress (CRS) and the underlying neuroprotective mechanisms.Methods Adult male Kunming(KM) mice, weighing 20 ~25 g, were restrained in wellventilated Plexiglass tubes for 6 h daily beginning from 10: 00 to 16 : 00 for 21 consecutive days.Mice were injected with retigabine (10 mg· kg1) or vehicle (10% DMSO) 30 min before restraint stress for 21 days.After stressor cessation, the spatial learning and memory was determined by Morris water maze test, the levels of pAkt, pGSK3β and pErk1/2 of hippocampal tissues were examined by western blot.Results Compared with control group, CRS mice exhibited significantly longer escape latencies on day 2, 3 and 4 (P <0.05, P <0.01, P <0.01) respectively, but retigabine (10 mg · kg1) treatment had no influences on escape latencies compared with CRS group.During the probe test, CRS mice spent significant less time in target quadrant than control group (P <0.01).Compared with CRS group, retigabine (10 mg · kg1)treatment increased the time spent in target quadrant (P <0.01).Additionally, the swimming speed showed no significant differences among groups.Western blot results showed that the levels of pAkt, pGSK3β and pErk1/2 in the hippocampus of CRS mice were significantly decreased compared with control group.Compared with CRS group, retigabine (10 mg· kg1) treatment strongly prevented the reduction of pAkt and pGSK3β (P < 0.01),but had no effect on the reduction of pErk1/2.Conclusion Retigabine protected against CRSinduced spatial memory retrieval impairment partly via activation of Akt/GSK3β signaling pathway.
其他文献
目的：以症状性大脑中动脉(middle cerebral artery, MCA)斑块的活体高分辨率磁共振(high-resolution magnetic resonance imaging,hrMRI)数据为基础,建立3D完全耦合的流-固耦合(fluid-structure interaction,FSI)模型,探索不同组分的MCA斑块的生物力学特征。
目的 探讨双能量CT血管造影(dual-energy computed tomography angiography,DECTA)在夜间急性自发性脑出血诊治中的应用价值.方法 回顾性分析总结249例夜间急性自发性颅内出血患者的双能量CTA影像学资料,结合数字减影血管造影术(DSA)和手术所见进行回顾性分析.结果 249例夜间急性自发性脑出血患者中DECTA发现142例(57.03％,142/249
目的 探讨颌面部血管瘤的颈外动脉造影诊断及血管内介入治疗的价值。方法 25例颌面部血管瘤患者，采用Seldinger技术行一侧股动脉穿刺插管，先行患侧颈外动脉主干侧位造影，明确颌面部血管瘤的具体部位、瘤体大小、供血主源，有无动脉瘤或动静脉瘘存在，将微导管逐一超选择插入供血动脉支内合适位置处(微导管尖端尽可能深、无嵌顿不阻断血流、无正常供血分支显示)，以PVA颗粒(500～750μm)栓塞，造影复查
目的 探讨颅骨及颌面骨骨纤维异常增殖症的CT表现及其特征。资料与方法 以多层螺旋CT平扫、增强扫描并三维重建技术对平片所示颅骨及颌面骨病变、拟诊良性骨病病例进行检查,结合手术病理资料,进行回顾性分析。结果 经手术病理证实为骨纤维异常增殖症共15例,其中发生于颅骨5例,发生于颌面骨8例,发生于筛骨2例。单骨发病、单发病灶3例,多骨受累、多发病灶12例。依其骨质破坏类型及CT密度改变程度,其中硬化型8
目的 探讨颅脑原始神经外胚层肿瘤的CT及MRI影像学表现,以提高对本病诊断水平.方法 回顾性分析11例经病理证实的颅内原始神经外胚层肿瘤的临床及CT、MRI等影像表现.结果 11例颅内原始神经外胚层肿瘤的平均年龄为33岁,年龄最小的5岁,年龄最大的69岁,其中6例在30岁以下,11例中男性占7例.11例病人中,顶叶、枕叶、顶枕叶各1例,颞叶及额颞叶各2例,额叶4例.11例病人中均以不同程度头痛(1
目的：探讨临床上视神经脊髓炎(neuromyelitis optica,NMO)患者多次MRI随访过程中的动态影像学表现特点.材料与方法：收集临床诊断明确、病例资料完整的NMO患者共5例(均为女性,年龄16-49岁,平均年龄为26.6±13.1岁),对照其临床治疗经过和详细病程,回顾性观察、分析相应时期的多次MRI检查资料.结果：5例NMO患者均出现过视神经损伤、视力下降等视神经炎表现,NMO患者
Aim Retinoic acid receptor alpha (RARα) plays a critical role in the differentiation process of osteosarcoma cells induced by alltrans retinoic acid (ATRA).However, degradation of RARα through ubiquit
Aim To study the influence of SiniSan on the Tryptophankynurenine (TRPKYN) pathway, the activity and content of the key metabolism enzyme indoleamine2, 3dioxygenase (IDO).Methods The mice model was es
Aim To investigate the effect of clonidine on anxietylike behaviors of rats subjected to chronic hypoperfusional cerebral ischemia.Methods Chronic hypoperfusional cerebral ischemia was established by
Depression is a leading cause of disability worldwide.However the biological and molecular mechanisms underlying this disorder remain to be unknown.Stress is a disposing factor in the development of d