An “off-on” fluorescence probe for specially recognize on Cys and its application in bioimaging

• 作 者: YinCaixia[1]YangYutao[1]HuoFangjun[2]
• 机 构: Institute of Molecular Science,Shanxi University 030006,Shanxi Taiyuan
• 出 处: 中国化学会第29届学术年会
• 发表日期: 2014年期

　　Thiols play essential roles in biological systems.As im-portant biothiols,cysteine(Cys),homocysteine(Hcy),and glutathione(GSH)which have the similar structures play crucial roles in human physiology.Their abnormal levels are linked to a number of diseases.Up to date,the design of highly selective probes for Cys,Hcy,and GSH is still a challenge and very few examples have been reported so far.Particularly,only a few sensors can detect Cys over Hcy and GSH in biological sys-tems12.We herein report an “off-on” fluorescent probe for specially recognize on Cys based on N-[4-Methylcoumarin-7-yl] malei-mide.For specific recognition on Cys of probe depends on its maleimide group,the structural and acidic differences of bio-thiol itself.Furthermore,the ability of probe to detect Cys in living cells(HepG2 cells)via an enhancement of the fluores-cence was proved.