Identification of early diagnostic antigens from Trichinella spiralis muscle larval excretory:secret

• 作 者: Li Wang Jing Cui Dan Dan Hu Ruo Dan Liu Zhong Quan Wang
• 机 构: Department of Parasitology,Medical College,Zhengzhou University,Zhengzhou 450052,China
• 出 处: 中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
• 发表日期: 2013年7期
• 关键词: Trichinella spiralis muscle larvae excretory:secretory proteins early diagnostic

　　The excretory: secretory (ES) proteins of Trichinella spiralis muscle larvae (ML) come mainly from the excretory granules of the stichosome and the cuticles (membrane proteins),are directly exposed to the host immune system,and are the main target antigens which induce the immune responses.Although the ES proteins are the most commonly used diagnostic antigens for trichinellosis,their main disadvantage is the false negative results during the early stage of infection.The aim of this study was to identify early specific diagnostic antigens in T.spiralis ES proteins.The ES proteins were analyzed by two:dimensional electrophoresis (2:DE),and more than 33 protein spots were detected with isoelectric point (pI) varying from 4 to 7 and molecular weight from 40 to 60 kDa.When probed with sera from mice infected with T.spiralis at 18 day post:infection,21 protein spots were recognized and then identified by MALDI:TOF/TOF:MS.The protein spots identified were characterized to correlate with four different proteins,including two serine proteases,one deoxyribonuclease (DNase) Ⅱ,and one putative trypsin.The four proteins were functionally categorized into molecular function and biological process according to GO hierarchy.For the molecular function category,four proteins had catalytic and hydrolase activity.In the biological process category,all of the four proteins were also related to metabolic process.These four proteins in T.spiralis ES proteins might be the early specific diagnostic antigens for trichinellosis.