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目的:探讨钙离子能否跨缝隙连接在内皮细胞与平滑肌细胞间进行传递。方法:采用植块法分别培养大鼠主动脉内皮及平滑肌细胞,将内皮细胞与平滑肌细胞分别接种至Transwell insert膜的两侧,观察两种细胞在Transwell insert膜两侧生长情
Objective: To investigate whether Ca2 + can cross the gap between endothelial cells and smooth muscle cells. Methods: The aorta endothelial cells and smooth muscle cells were cultured by explant method. The endothelial cells and smooth muscle cells were inoculated on both sides of Transwell insert, respectively. The growth of both cells was observed on both sides of Transwell insert