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前言 Epigenetic mechanism has been suggested to explain the effects of the maternal diet on the development of disease in offspring.But few studies have made to certificated it.目的 The objective of this study was to find the mechanism underlying the global decreasing gene expression we have observed in early study.方法 In the present study,female C57BL/6j mice were fed with chow or a high lipid and high energy diet(HLE)during gestation and lactation.After weaning,all the offspring were fed with chow diet until 10 weeks old when they were killed and liver samples were gathered.The global DNA methylations were measured by kits and Arrays provided by Affymetrix Company.The expressions of selected genes related to lipid and glucose metabolism were measured by western blot and RT-PCR.结果 The results indicated that the percent of methylated DNA in offspring from dams fed with HLE diet was significantly higher than that from chow diet and most of these genes were hypermethyalted on promoter regions.The nuclear protein content and mRNA levels of hypermethylated genes,such as PPARγ and LXRα,were decreased significantly in HLE group in offspring.结论 The results suggested that DNA methylation change might be the mechanism of the maternal diet regulating the expression of genes of offspring.