Molecular characterization of Cryptosporidium in bats from Yunnan Province,Southwestern China

• 作 者: Wei Wang Lili Cao Biao He Jiping Li Quan Liu Quanshui Fan
• 机 构: Military Veterinary Institute,Academy of Military Medical Sciences,Key Laboratory of Jilin Province
• 出 处: 中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
• 发表日期: 2013年7期
• 关键词: Cryptosporidium bats genotype China nested PCR

　　The present study was conducted to investigate the prevalence and genotypes of Cryptosporidium in bats.A total of 247 bats,belonging to Rhinolophus sinicus,Rousettus leschenaultia,Aselliscus stoliczkanus,and Hipposideros fulvus,were collected in Yunnan Province,Southwestern China,and the intestinal tissues were examined for Cryptosporidium infection by PCR amplification of the small subunit ribosomal RNA (SSU rRNA).The overall infection rate was 7.7% (95% CI,4.5% to 11.0%),with R.sinicus having the highest level at 9.5% (95% CI,2.8% to 16.1%),followed byA.stoliczkanu at 7.8% (95% CI,2.2% to 18.9%),H.fulvus at 7.2% (95% CI,1.1% to 13.4%),and R.leschenaultia at 5.7% (95% CI,1.2% to 15.7%).DNA sequence and phylogenetic analyses based on SSU rRNA revealed the presence of two novel genotypes,designated as Cryptosporidium bat genotype Ⅰ in A.stoliczkanus and R.sinicus,and Cryptosporidium bat genotype Ⅱ in R.leschenaultia,R.sinicus,and H.fulvus.This is the first report of Cryptosporidium genotypes in bats.Further biological and genetic eharacterisation is needed to determine the relationship of the two novel genotypes to established Cryptosporidium species/genotypes.