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目的采用化学与生物物理方法测量肌质网功能时,由于肌质网制备过程改变其生理环境而不能真正反应其功能。本实验拟建立观测肌纤维肌质网钙离子释放功能的简便方法。方法采用大鼠离体比目鱼肌肌条灌流,分析间断强直收缩
Objective To measure the function of sarcoplasmic reticulum by chemical and biophysical methods, the sarcoplasmic reticulum can not really change its function due to its physiological environment. This experiment to establish a simple method to observe the muscle fiber sarcoplasmic reticulum calcium release. Methods Rat isolated muscle strips of soleus muscle were used to analyze the intermittent tetanic contraction