【摘 要】
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OBJECTIVE:To study the regulating effect of Shuganjianpifang(SGJP)in CD4+CXCR5+T cells over the immune microenvironment of hepatocellular carcinoma(HCC).METHODS:Forty mice of C57BL/6 type were divided
【机 构】
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Oncology Department, Clifford Hospital of Guangzhou University of Chinese Medicine,Guangzhou 511495,
【出 处】
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世界中联自然疗法研究专业委员会第五届学术年会暨国际热疗医学学会第35届学术年会
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OBJECTIVE:To study the regulating effect of Shuganjianpifang(SGJP)in CD4+CXCR5+T cells over the immune microenvironment of hepatocellular carcinoma(HCC).METHODS:Forty mice of C57BL/6 type were divided into blank group,model group,SGJP group and cisplatin(DDP)group as random number table method.The model group,SGJP Group and cisplatin group were inoculated with H22 liver cancer cells,and intervened with SGJP after 48h.The control group was treated with cisplatin by intraperitoneal injection.Those mice were sacrificed after fourteen days,then their tumor cells were transplanted by HE staining for immunohistochemical analysis;after we run through isolation of splenic lymphocytes,CD4+CXCR5+T cells were detected by flow cytometry;the levels of IL-21,IL-6,IFN-γ and CXCR5 in peripheral blood were detected by enzyme linked immunosorbent assay(ELISA);The expression of IL-21,IL-6 and CXCR5 genes in transplanted tumor was analyzed by reversing transcription-reverse transcription(RT-PCR).RESULTS:SGJP has an inhibitory effect on transplanted tumor.SGJP can improve the content of serum IL-6,IL-21,INF-gamma in transplanted tumor mice,and has statistical significance.CONCLUSION:SGJP improves CD4+CXCR5+T cells amount and function,which in return improves the tumor immune microenvironment of HCC.
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