论文部分内容阅读
Obiective To construct multifunctional nanoparticles and to evaluate its properties of Fluorescence imaging(FLI),magnetic resonance imaging(MRI)and targeting drug delivery in vivo.Methods Superparamagnetic iron oxide(USPIO)nanoparticles coated with porous silica and 3-aminopropyltriethoxysilane(APTES)were synthesized(USPIO@mSiO2-NH2).The fluorescein isothiocyanate(FITC)and the targeting molecule folic acid(FA)were modified by carbodiimide to form FLI and MRI bifunctional nanoprobe(USPIO@mSiO2-FA-FITC).Physical properties are characterized by TEM,XRD,XPS,Zeta-patential,etal.MTT assay was performed to investigate the potential toxicity of the USPIO@mSiO2-FA-FITC.MRI signal strength variation was detected by 3.0T MRI,and the FLI ability of the nanocomposite was evaluated by confocal laser scanning microscopy after USPIO@mSiO2-FA-FITC with Fe concentration 2.5 μmol/L incubating human SGC7901 cell for 60 minutes.the ability of USPgastric cancer.Result: The USPIO@mSiO2-FA-FITC nanoparticles showed excellent stability in PBS.The average diameter of the USPIO@mSiO2-FA-FITC nanoparticles was approximately 55 nm,and the hydration kinetics particle size of USPIO@mSiO2-FA-FITC nanoparticles was about 115 nm.The bore diameter of mSiO2 was about 2.6 nm.In vitro studie,the USPIO@mSiO2-FA-FITC nanoparticles showed a better specificity by FLI and rather lower toxicity of human SGC7901 cell via MTT assay.The MRI showed the T2 signal intensity of USPIO@mSiO2-FA-FITC nanoparticles group was lower than control group,and the experimental group was 3.2 times than the control group in vivo.The nanoparticles had better drug delivery and treatment ability for l imaging and drug delivery ability in vitro and in vivo.Result The USPIO@mSiO2-FA-FITC nanoparticles showed excellent stability in PBS.The average diameter of the USPIO@mSiO2-FA-FITC nanoparticles was approximately 55 nm,and the hydration kinetics particle size of USPIO@mSiO2-FA-FITC nanoparticles was about 115 nm.The bore diameter of mSiO2 was about 2.6 nm.In vitro studie,the USPIO@mSiO2-FA-FITC nanoparticles showed a better specificity by FLI and rather lower toxicity of human SGC7901 cell via MTT assay.The MRI showed the T2 signal intensity of USPIO@mSiO2-FA-FITC nanoparticles group was lower than control group,and the experimental group was 3.2 times than the control group in vivo.The nanoparticles had better drug delivery and treatment ability for l imaging and drug delivery ability in vitro and in vivo.Conclusion The multifunctional nanoparticles was succesfully synthesized.The nanocomposite was with bimodaIO@mSiO2-FA-FITC as a potential contrast agent was evaluate in nude mice.Subsequently anticancer drug doxorubicin(DOX)can be loaded inside the nanocomposite,obtaining an multifunctional nanoparticles(Fe3O4@mSiO2-DOX-FA-FITC).