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Labeling the interior of single virus is a powerful tool for monitoring the whole journey of virus.Owing to the inaccessibility of nucleocapsid packed by the envelope, labeling of the interior of virus has always been a challenge.Quantum dots (QDs) with an excellent fluorescent properties, have been widely used for labeling the exterior of virus for single-virus tracking.Herein we proposed a strategy to label the nucleocapsid of baculovirus with QDs.