Regulation of retromer-motor interaction by PI(4)P: a molecular mechanism for dynein cargo release a

• 作 者: YangNiu;ChengZhang;ZhiHong;KeLi;DemengSun;ZheSun;ChanglinTian;WeiminGong;Jia-JiaLiu;
• 机 构: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Bio
• 出 处: 第四届细胞结构与功能的信号基础研讨会
• 发表日期: 2012年期

　　The molecular mechanisms for the retrograde motor dynein/dynactin to unload its cargoes at their final destination remain to be elucidated.In this study, we have investigated the regulatory mechanism underlying release of retromer-associated cargoes at the trans-Golgi network (TGN).Here we report that PI(4)P, a Golgi-enriched phosphoinositide, negatively regulates the protein-protein interaction between the p150Glued subunit of dynein/dynactin and the retromer component SNX6.We show that PI(4)P specifically facilitates dissociation of retromer-mediated membranous cargoes from the motor at the TGN and uncover an important function for PI(4)P in the precise spatial control of retrograde vesicular trafficking to the TGN membrane.In addition, PI(4)P also regulates SNX4-mediated vesicular trafficking to the endocytic recycling compartment by modulating its interaction with dynein.These results establish organelle-specific phosphoinositide regulation of motor-cargo interaction as a mechanism for cargo release by molecular motors at target membrane.