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INTRODUCTION: Platelet Lysate(PL)contains a cocktail of growth factors and cytokines,which actively participates in tissue repair.In previous publications,we have shown that PL transiently increases the inflammatory response occurring in wound while induces proliferation of resident cells in keratinocyte and osteoblast systems.The aim of this study was to assess the activity of PL on endothelial cells,the first cells that respond to the wound,causing damage of blood vessels and resulting in the cascade of coagulation and release of platelet content.METHODS: Human Umbilical Vein Endothelial Cells(HUVEC)were obtained from primary cultures derived from fresh umbilical cords and cultured on gelatin coated plates in M199 medium supplemented with 10%FCS,10 μg/l aFGF,10 μg/L bFGF,10 μg/l EGF,1 mg/L hydrocortisone.PL was produced from platelet rich plasma obtained from a pool of human blood donors.Platelets were washed and re-suspended in phosphate-buffered saline at a concentration of 10 millions platelets/μl and subjected to 3 freeze/thaw cycles followed by a high-speed centrifugation.The supernatant,was added to complete culture medium at a final concentration as 5%.RESULTS: PL inhibits the inflammatory response induced by IL-1 by inhibiting the NF-κB activation and repressing the secretion of IL-6 and IL-8.PL increases the proliferation of endothelial cells and induces proliferation in quiescent cells by activating the pathways of ERKs and AKT,and the synthesis of Cyclin D1.CONCLUSIONS: PL has a protective activity towards endothelial cells by inhibiting the response to inflammatory cytokines possibly released in vessels damage.PL is able to induce in quiescent cells a proliferative response,important for vessel repair indicating a possible mechanism in the restoration of blood vessel functional activity in wound healing.