RNA-Seq analysis of gynoecious and weak female cucumber revealing cell cycle pathway may regulate se

• 作 者: Rui Wang Yu'e Lin Qingmin Jin Yujuan Zhong Chunpeng Yao Zhaojuan Liang Tingquan Wu
• 机 构: Vegetable Research Institute,Guangdong Academy of Agricultural Sciences;Guangzhou,510640,China
• 出 处: 广东省园艺学会第十一届会员大会暨广东园艺产业创新与发展学术研讨会
• 发表日期: 2017年3期

　　To investigate key genes involved in molecular regulatory networks of cucumber sex determination,we performed a genome-wide high-throughput RNA sequencing for young apical buds of gynoecious and weak cucumber at three growth stages(one-leaf one-bud,three-leaf one-bud,five-leaf one-bud).Seven comparisons from the same cultivar at three different stages and at the same stage between the two cultivars were analyzed and the results showed that compared with differentially expressed genes in weak cucumber,more genes were upregulated at one-leaf one-bud stage,and downregulated at three-leaf one-bud stage in gynoecious cucumber.In addition,there were four kinds of gene expression trends(0,1,6,7)enriched significantly in gynoecious cucumber,and only two kinds of gene expression trends(5,6)enriched significantly in weak cucumber.Together with the data of GO,pathway,gene expression trends and qPCR,9 genes were identified and considered as candidate genes that may be involved in sex differentiation regulation in cucumber.These genes included Cs-MCM6,Cs-ACT3,Cs-XRCC4,Cs-MCM2,Cs-CDC45,Cs-Dpri,Cs-H2B,Cs-CDC20 and Cs-CNGCl,and among them,there are 5 genes(Cs-MCM6,Cs-MCM2,Cs-CDC45,Cs-Dpri,Cs-CDC20)involved in cell cycle pathway,suggesting that cell cycle pathway maybe play an important role in sex determination in cucumber.