基于共轭聚合物组合探针及FRET机制ATP检测研究

来源 :2016全国生命分析化学学术大会 | 被引量 : 0次 | 上传用户:hawkwangyan
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  ATP是细胞进行各种生化过程的第一能量来源,同时也是调节细胞运动、神经递质传递以及离子通道的重要信号分子,因此对ATP进行实时监测和定量分析具有重要意义.我们设计合成了一种侧链带有苯硼酸基团的新型中性水溶性共轭聚合物PPE-PBA,然后将阳离子水溶性共轭聚合物PFP-NMe3+与PPE-PBA相结合,基于聚合物之间的FRET机理构建了一种双共轭聚合物荧光探针来检测ATP.
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