Expressions of the imprinted genes from cluster Dil1-Dio3 in corticospinal projection neurons

• 作 者: CSQU;YJYANG;HPTAO;JQU;JGCHEN;
• 机 构: School of Ophthalmology and Optometry, Wenzhou Medical Collage, the Key Laboratory of Visual Science
• 出 处: 中国神经科学学会第九届全国学术会议暨第五届会员代表大会
• 发表日期: 2011年期

　　The corticospinal projection neurons (CSPN), originated from the layer Ⅴ of cerebral cortex, generate neural impulses and control execution of movement.Malfunction of CSPN give rise to upper motor neuron (UMN) syndrome.To identify the molecules of the motor neuron related diseases, we labeled the CSPN of postnatal mice by a retrograde tracer Red Beads, and isolated them by laser capture micro-dissection (LCMD).Started with gene expression screening, we found that maternally expressed gene 3 (Meg3) and Meg8 were highly enriched in CSPN during the early development.Meg3 and Meg8 are colocalized with Ctip2, a marker of the layer V.Meg3 and Meg8 are from a transcription unit located in the imprinted cluster Dil 1-Dip3, whereas a paternally expressed gene Rtll is known in the opposite strand.Rt11 had a minimal expression in the cortex as determined by in situ hybridization.