Isolation and function analysis of floral asymmetry genes in Coriandrum sativum (Apiaceae)

• 作 者: SONGChun-feng;LIUQi-xin;
• 机 构: Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing 210014, China
• 出 处: 第五届长江三角洲地区植物学学术研讨会
• 发表日期: 2014年期

　　Floral asymmetry is controlled by multiple genes.Among of them, CYC-like gene belong to TCP transcription gene family and the function of it is relatively conserved in angiosperm: responsible for dorsal-ventral asymmetry of flowers.Inflorescence of Apiaceae (Rosidae) is typical umbellate, composed by many umbellets.Among one umbellet, florets in the central are actinomorphic flowers, while florets in the margin are zygomorphic flowers.Apiaceae is one unique material for floral asymmetry.3 CYC-like genes of Coriandrum sativum, one representative plant in Apiaceae, were cloned with RACE-PCR.The function of them were studied by Real-time PCR, Tissue in situ hybridization and Transgene.The results showed that the function of CYC-like genes were conserved in Apiaceae too.Among the genes, the expression of CsCYC2 and CsCYC3 were only detected in the dorsal region of zygomorphic flowers.The two genes were responsible for the dorsal-ventral asymmetry of florets in Apiaceae.But the funcion of them were only limited on petals, not on five stamens.