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The colon is one of the bodys metabolically most active organs.While insight in colonic microbial diversity increases, little is known of the metabolic function of the microbes, the way diet affects this, and how the metabolites affect obesity or gut health.13C-stable isotope labeling of substrates was employed with the aim to investigate the activity of the colonic microbiota in detail.Three unifonnly-13C-labeled model substrates were used: starch, inulin and lactose.