【摘 要】
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Research purpose We want to know the specific mechanism of plasmodium specific CD4 + T cells activation in the whole killed blood stage vaccine.Methods Firstly, C5aactivation in both wild type and C5a
【机 构】
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Department of Pathogenic Biology, Third Military Medical University
【出 处】
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中华医学会第六次全国艾滋病、病毒性丙型肝炎暨全国热带病学术会议
论文部分内容阅读
Research purpose We want to know the specific mechanism of plasmodium specific CD4 + T cells activation in the whole killed blood stage vaccine.Methods Firstly, C5aactivation in both wild type and C5aRknockout (C5aR-/-) BALB/c was determined.Secondly, wild type and C5aR-/-BALB/c Jacksonlaboratory) were immunized with 1.5 × 107 pRBC lysate admixed thoroughly with aluminium hydroxide Pierce), and challenged with 1 × 106 Plasmodium yoelii (P.y) 17XL pRBC at two weeks post final immunization.Then, both parasitemia and survival rate in both immunized mice were determined.The level of malaria parasite-specific IgG (Sigma-Aldrich) in serum and the spleen CD4 + T cell function were detected in both immunized mice by ELISA, CFSE-based proliferation and FACS, respectively.Finally, splenic DCs maturation and their ability to allostimulate CD4 + T cells of both immunized mice were also investigated by FACS and mixed lymphocyte reaction (MLR).
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