Identification of an endogenetic biocontrol strain NS03 and its efficacy in controlling pomegranate

• 作 者: Ma Yao-hua Tan Xiao-yan
• 机 构: College of Life Science, Zaozhuang University, Zaozhuang, China
• 出 处: 中国植物病理学会第十一届青年学术研讨会
• 发表日期: 2013年11期
• 关键词: antagonistic endophytic bacteria Bacillus subtilis biological character Zythia v

　　The pomegranate fruit dry rot (Zythia versoniana Sacc.) is the economically important diseases in pomegranate production worldwide.Fungicides are overused in controlling this disease and might result in chemical residues in pomegranate plants and their environments which are not ecofriendly.To solve these problems, an intensive study on biocontrol of pomegranate dry rot disease is needed.An antagonistic entophytic bacterial strain NS03 with strong antifungal activity against Z.versoniana was identified as Bacillus subtilis based on its 16S rDNA sequence analysis and construction of phylogenetic tree as well as physiological and biochemical characters.In vitro and Laboratory tests indicated the control efficacy of NS03 was obvious and the inhibition rate reached 43.27% and 99.51%, respectively.The experimental data indicated that the entophytic antagonistic strain NS03 was a promising biocontrol agent against the pomegranate dry rot disease.