【摘 要】
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Aim: To evaluate the protective efficacy of a DNA vaccine encoding T.gondii ROP5 and GRA15 antigens.Methods: We constructed eukaryotic plasmids expressing pVAX-ROP5 and pVAX-GRA15,and measured the imm
【机 构】
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State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of
【出 处】
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中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
论文部分内容阅读
Aim: To evaluate the protective efficacy of a DNA vaccine encoding T.gondii ROP5 and GRA15 antigens.Methods: We constructed eukaryotic plasmids expressing pVAX-ROP5 and pVAX-GRA15,and measured the immune responses to these DNA vaccines.Results: Kunming mice immunized with pVAX-ROP5 or pVAX-GRA15 showed significantly increased serum IgG2a titers;Th1 responses association with the production of IFN-γ,IL-2,IL12 p40 and IL-12 p70;cell-mediated cytotoxic activity with increased frequencies of IFN-γ secreting CD8+T cells(CD8+ IFN-γ+ T cells),as well as prolonged survival time(19.4±4.9 days for ROP5;17.8±3.8 days for GRA15)and brain cyst reduction(57.4%for ROP5;65.9%for GRA15)compared to control mice.Co-administration with pVAX-ROP5 and pVAX-GRA15 boosted the cellular and humoral immune responses,and significantly increased cyst reduction(79%)and prolonged the survival of immunized mice(22.7±7.2 days).Conclusion:Co-immunization of pVAX-ROP5 and pVAX-GRA 15 increase the protective efficacy.
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