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Amylosucrase (AS) (E.C.2.4.1.4.) from Neisseria polysaccharea was applied to form polysaccharides with low digestibility.Amylosucrase catalyzes a transglycosylation reaction to produce α-l,4-glucan using sucrose as a unique substrate.Particularly,in the presence of an acceptor,it catalyzes the elongation of some external chains at their non-reducing end