论文部分内容阅读
目的:归纳Glut-1及其调控因子HIF-1α与P53在卵巢癌组织中免疫组化染色阳性表达的的规律,探讨其与临床病理参数的相关性,分析Glut-1在诊断中应用的价值并推测缺氧在卵巢癌发病中的作用。方法:免疫组织化学EnVision法检测Glut-1、HIF-1α及P53在63例卵巢癌组织中的表达,采用SPSS统计分析软件进行相关性分析。结果:在卵巢癌组织中,(1)Glut-1呈弥漫性、局灶性或散在性多种分布模式,且随染色评分的增加,其在细胞膜上的表达愈发突出,并与HIF-1α阳性表达区域部分重叠。(2)Glut-1的表达与HIF-1α、P53有相关性,呈序列正相关。(3)Glut-1表达与组织学分级、Kurman分型均呈序列正相关,与FIGO分期无相关性;P53表达仅与Kurman分型有相关性,与组织学分级、FIGO分期无相关性;HIF-1α则与组织学分级、Kurman分型及FIGO分期均无相关性。(4)卵巢低级别与高级别浆液性癌中Glut-1与P53的表达均有显著不同,且Glut-1的表达强度与卵巢浆液性癌的分级呈正相关,而HIF-1α的表达与分级无关。结论:不同亚型的卵巢癌,其微环境缺氧状态不同,Glut-1与P53联合检测是有助于指导卵巢癌分型及浆液性卵巢癌分级的指标。
OBJECTIVE: To summarize the regularity of the positive expression of Glut-1 and its regulatory factors HIF-1α and P53 in ovarian cancer tissues and to explore the relationship between Glut-1 and clinicopathological parameters, and to evaluate the value of Glut-1 in diagnosis And speculated hypoxia in the pathogenesis of ovarian cancer. Methods: EnVision immunohistochemistry was used to detect the expression of Glut-1, HIF-1α and P53 in 63 cases of ovarian cancer. SPSS statistical software was used to analyze the correlation. Results: In ovarian cancer, (1) Glut-1 showed diffuse, focal or scattered patterns. With the increase of staining score, the expression of Glut-1 in the cell membrane became more prominent, 1α positive expression area partially overlap. (2) The expression of Glut-1 was correlated with HIF-1α and P53, and was positively correlated with the sequence. (3) Glut-1 expression was positively correlated with histological grade and Kurman classification, but not with FIGO stage. P53 expression was only correlated with Kurman classification, but not with histological grade and FIGO stage. HIF-1α had no correlation with histological grade, Kurman classification and FIGO staging. (4) The expression of Glut-1 and P53 were significantly different between low grade and high grade serous ovarian cancer, and the expression intensity of Glut-1 was positively correlated with the grade of ovarian serous carcinoma, while the expression and grade of HIF-1α Nothing to do CONCLUSION: The ovarian cancer with different subtypes has different microenvironment and hypoxia status. Combined detection of Glut-1 and P53 is helpful to guide the classification of ovarian cancer and serous ovarian cancer.