【摘 要】
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To date, there are about 100 modified nucleosides (MNs) have been identified in RNA species and the majority of them were found in tRNAs.Except their common
【机 构】
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School of Bioengineering, Dalian Polytechnic University, 116034, China
【出 处】
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第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
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To date, there are about 100 modified nucleosides (MNs) have been identified in RNA species and the majority of them were found in tRNAs.Except their common properties as components of RNAs, MNs have been confirmed to be of multifunctional.Some researches had demonstrated MNs involvement in anti-stress responses in the yeast.Gene yfcC was recently found to modulate anaerobic survival of Escherichia.coli (E.coli) by us.In order to determine if disruption ofyfcC could stimulate certain response manifesting by MNs, we developed a quick and simple method to quantify 20 nucleosides by using triple quadurupole liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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