【摘 要】
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Background: Cell proliferation and turnover are fueled by stem cells.In a previous study, we demonstrated that rat eccrine sweat glands contained abundant bromodeoxyuridine (BrdU)-label retaining cell
【机 构】
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Second Affiliated Clinical Hospital,Shantou University Medical School,Shantou,Guangdong,515041
【出 处】
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首届全国组织修复与再生医学大会暨国际组织修复与再生医学学术研讨会
论文部分内容阅读
Background: Cell proliferation and turnover are fueled by stem cells.In a previous study, we demonstrated that rat eccrine sweat glands contained abundant bromodeoxyuridine (BrdU)-label retaining cells (LRCs).However, morphological observation showed that eccrine sweat glands usually showed little or no signs of homeostatic change.Objective: In this study, we account for why the homeostatic change is rare in eccrine sweat glands based on cytokinetic changes in BrdU-LRCs turnover, and we also determine the BrdU-labeled cell type.Methods: Thirty-six newborn SD rats, were intraperitoneally injected with 50 mg/kg BrdU twice daily at a 2 h interval for 4 consecutive days.After a chase period of 4, 6, 8, 12, 24 and 32 weeks, rats were euthanized,and the hind footpads were removed and processed for BrdU immunostaining, and BrdU/?-SMA and BrdU/CK14 double-immunostaining.BrdU-LRCs were observed in the ducts, secretory coils and mesenchymal cells in all survival time points.Results: The percentage of BrdU+ cells in rat eccrine sweat glands averaged 4.2?1.2 % after 4 weeks of chase, increased slightly by the 6th week, averaging 4.4?0.9 %, and peaked at 8 weeks, averaging 5.3?1.0 %.Subsequently, the average percentage of BrdU+ cells declined to 3.2?0.8 % by the 32nd week.There was no difference in the percentage of BrdU-LRCs among the different survival time points except that there is a significant decrease in the percentage of BrdU-LRCs detected at 24 weeks versus 8 weeks, and 32 weeks versus 8 weeks.Conclusions: The BrdU-LRCs turnover is slow in eccrine sweat glands, which account for why there are little or no signs of homeostatic change in eccrine sweat glands.
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