Establishment of antigen capture ELISA detection method for H9subtype avian influenza virus HA prote

• 作 者: SunQuan-wen[1]WangJin-liang[2]YangCui-jun[1]GeJian[1]WangAi-Hua[1]XuTong[1]
• 机 构: Hebei North University, Zhangjiakou, China
• 出 处: 中英禽病国际研究中心成立典礼暨中英禽病最新研究进展研讨会
• 发表日期: 2015年期

　　The objective of present research was to establish antigen capture ELISA detection method for H9 subtype avian influenza virus.BALB/c mice were immunized with HA recombination protein, and then the mouse splenic cells were fused with SP2/0 cells, hybridoma cell stably secreting anti-HA McAb was screened by ELISA coated with the AIV antigen, an antigen capture ELISA based on monoclonal antibody was developed for detection of H9 AIV.Hybridoma cell stably secreting anti-HA McAb designated 5Fl 0, the immunoglobulin type was IgG2b type with κchain.The H9 AIV AC-ELISA showed no cross-reaction with other five avian viruses (ILTV, NDV, EDSV,, IBV, and IBDV), the sensitivity eight times higher than HA test, Comparing with RT-PCR, the concordance was 94.8 %, the sensitivity was 97.1%, and the specificity was 94.4 %.The H9 AIV AC-ELISA has good specificity, sensitivity and repeatability, could be used for diagnosing H9 subtype AIV infections.