The effect of selenium deficiency on the DNA methylation in the tissues of chicks

• 作 者: ZHANGZiwei;YAOHaidong;LIShu;XuShiwen;
• 机 构: Department of Veterinary Medicine ,Northeast Agricultural University,Harbin 150030 , P.R.China
• 出 处: 中国畜牧兽医学会家畜内科学会2014年学术研讨会
• 发表日期: 2014年期

　　Selenium (Se), an essential trace element,was indicated to play important role in the regulation of DNA methylation.Se deficiency induced hypomethylation in tissue of mice.However, the effect of Se on DNA methylation in poultry was less reported.In the present study, two groups of day-old layer chicks (n =60/group) were fed a corn-soy basal diet (33 mg Se/kg;produced in the Se-deficient area of Heilongjiang, China) or the diet supplemented with Se (as sodium selenite) at 0.15 mg/kg for 55 d.Then the DNA methylation level, DNA methyltransferases (DNMTs),Demethylase (MBD2) were examined.The results indicated that DNA methylation level were lower in muscle tissues, brain tissues, immune tissues and livers of chicks in Se deficiency groups than that of control group (P < 0.05).Se deficiency decreased the mRNA levels of DNMT1、 DNMT3A、DNMT3B, but increased MBD2 in muscle tissues, brain tissues, immune tissues and livers of chicks (P < 0.05).It indicated that Se deficiency influenced the level of DNA methylation in chicks.