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Galacto-oligosaccharides (GOS),the beta-linked carbohydrate compounds containing galactose and glucose,are prebiotics derived from lactose catalyzed with beta-galactosidases.GOS are applied in functional dairy products,and stimulation of growth of probiotic lactobacilli and bifidobacteris in human gut.Thermophilic enzymes have industrial application value with the advantages of high reaction velocity,thermostability and less risk of contarnination.