Novel Polymorphisms of the APOA2 Gene and its Promoter Region Affect Body Traits in Cattle

• 作 者: YangZhou;CaixiaLi;HanfangCai;YaoXu;XianyongLan;ChuzhaoLei;HongChen;
• 机 构: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricu
• 出 处: 第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会
• 发表日期: 2013年期

　　Apolipoprotein A-Ⅱ (APOA2) is one of the major constituents of High-density lipoprotein and plays a critical role in lipid metabolism and obesity.However, similar research for the bovine APOA2 gene is lacking.In this study, polymorphisms of the bovine APOA2 gene and its promoter region were detected in 1021 cows from four breeds by sequencing and PCR-RFLP methods.Totally, we detected six novel mutations which included one mutation in the promoter region, two mutations in the exons and three mutations in the introns.There were four polymorphisms within APOA2 gene were analyzed for the low frequencies of other two mutations.The allele A, T, T and G frequencies of the four loci were predominant in the four breeds when in separate or combinations analysis which suggested cows with those alleles to be more adapted to the steppe environment.The association analysis indicated three SVs in Nangyang cattle, two SVs in Qinchun cattle and the 9 haplotypes in Nangyang cattle were significantly associated with body traits (P<0.05 or P<0.01).The results of this study suggested the bovine APOA2 gene is possible a strong candidate gene for body traits in the cattle breeding program.