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System peaks appear in HPLC, when the composition of the injected sample deviates from the mobile phase provided that there is a mutual interaction between a mobile phase component and a sample compound, for example a competition for adsorption sites.The system peaks can be used in the interpretation of separation mechanisms and in studies of solid phase adsorptions.In case of large disturbances at the injection the large system peaks created can interfere in the separations by inducing strange peak shapes.The system peaks can also be used to an advantage since gradients are created at the borders of the system peak and may give compressed efficient analyte peaks, i.e.improving the limit of detection.System peaks are generally developed in coupled column systems, since the transferred fractions from the first column most often strongly deviates from the composition of the second mobile phase.System peaks may also appear in capillary electrophoresis and they have been studied especially in systems developed for indirect detection with a marker present in the background electrolyte.