Polymorphisms of the Bovine DKK2 and Their Associations with Body Measurement Traits and Meat Qualit

• 作 者: XiaoliZhan[1]JianbinGao[1]YifanHuangFu[1]ChangzhenFu[1]LinsenZan[2]
• 机 构: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R.China
• 出 处: 第二届中国肉牛选育改良与产业发展国际研讨会暨中国畜牧兽医学会养牛分会八届二次学术研讨会
• 发表日期: 2013年期

　　The objective of this research were to detect bovine Dickkopf 2 (DKK2) gene polymorphism and analyze their associations with body measurement traits (BMT) and meat quality traits (MQT) of animals.Blood samples were taken from a total of 541 Qinchuan cattle aged from 18-24 months.Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was employed to find out DKK2 singlepolymorphism nucleotide (SNPs) and to explore their possible association with BMT and MQT.Sequence analysis of DKK2 gene revealed 2 SNPs (C29 T and A169C) in 5 untranslated region (5UTR) of exon 1.C29T and AI64T SNPs are both synonymous mutation, which showed 2 genotypes namely (CC, CT) and (AA and AC), respectively.Association analysis of polymorphism with body measurement and meat quality traits at the two locus showed that there were significant effects on CT, BL, RL, PBW, BFT, LMA, and IFC.These results suggest that the DKK2 gene might have potential effects on BMT and MQT in in Qinchuan cattle population and could be used for marker-assisted selection.