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When exposure of yeast cell to DNA-damaging agents result in DNA damage or synthesis block, RNR2 expression is induced via many gene regulation pathways.So RNR2 promoter was amplified by PCR from W303-1A yeast genosome and it was inserted into the upstream of the yeast report vector carrying the gene encoding a green fluorescent protein (yEGFP) optimised for yeast.Therefore the yEGFP expression is under the control of the RNR2 promoter in this constructed vector.The yeast strains was transformed with the this vector by lithium acetate method.In this way the yEGFP fluorescent yeast cell regulated by RNR2.It was found that the fluorescent density of the recombinant yeast cell had dose-effect relationships with the most of DNA alkylating agents, cleavage of DNA and inhibitors of DNA polymerases.These results indicate that this yeast cell can be used to screen many of DNA damage chemicals related to carcinogenesis.The assay can performed on 96-well microplates so it have the characteristics of rapid, convenience and high throughput.