Composite Tissue Allotransplantation Using Dendritic Cell Presensitization

• 作 者: SeokChanEun;RongMinBaek;HakChang;KyungWonMinn;SukWhaKim;
• 机 构: Department of Plastic Reconstructive Surgery Seoul National University College of Medicine Korea
• 出 处: 中国上海第七届国际新药发明科技年会
• 发表日期: 2009年期

　　Prevention of acute rejection in composite tissue allotransplantation without continuous immunosuppression lacks reports in worldwide literature.Need for chronic immunosuppression precludes the use of composite tissue allograft as a routine surgical reconstructive option.Recently dendritic cells (DC) gained considerable attention as antigen presenting cells that are also capable of immunologic tolerance induction.This study assesses the effect of alloantigen-pulsed DC in induction of survival increase in a rat hind limb allotransplantation model.Recipient blood derived mononuclear cells(MNC) were harvested from rat whole blood and cultured with GM-CSF and IL-4 for 2 weeks for DC generation.After recipient DC was co-cultured one day with donor mononuclear cells for presensitization,these donor-specific alloantigen pulsed dendritic cells were reinjected into tail vein before transplantation.The FK-506(tacrolimus) injection started immediately after transplantation and lasted for two weeks.Graft appearance challenges were assessed by histopathology and molecular biologic study.Donor antigen pulsed host dendritic cell combined with short-term immunosuppression agents protocol prolong hind limb composite allotransplantation survival and has potential therapeutic application for induction of donor antigen specific tolerance.