【摘 要】
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Objective: To assess the relationship between ERα methylation and MTA1 expression in breast cancer, and further to investigate whether or not the repressed ERα methylation could downregulate MTA1 expr
【机 构】
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Department of General Surgery,Department of Surgical Oncology,Department of Breast Surgery,the First
【出 处】
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22nd Asia Pacific Cancer Conference(第22届亚太抗癌大会)
论文部分内容阅读
Objective: To assess the relationship between ERα methylation and MTA1 expression in breast cancer, and further to investigate whether or not the repressed ERα methylation could downregulate MTA1 expression in vitro.Methods: Breast carcinoma tissue and paired non-tumor portion samples from female breast cancer patients (n=102)were collected from the archived tissue bank at the First Affiliated Hospital of China Medical University between2005 and 2011.We detected MTA1 protein expression by immunohistochemistry, and analyzed the correlation between MTA1 protein expression and the associated clinical pathological factors.ER1, ER3, ER4, and ER5 were chosen for the assay of methylation-specific polymerase chain reaction (MSP), thereby,analyzing the correlation between the ERα methylation and MTAlexpression.The cell lines were treated with 5-aza-2-deoxycytidine (5-Aza-dC) further to detect methylation status of the promoters, namely, ER1, ER3, ER4, and ER5, using Western blot and RT-PCR to detect MTA1 protein expression and mRNA after 5-Aza-dC treatment.Using Western blot and RT-PCR, MTA1 protein expression and mRNA level were detected after 5-Aza-dC treatment.
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