Function of increased human divalent metal transporter 1 in iron accumulation

• 作 者: Hua-MinXU;HongJIANG;LeiWANG;Jun-XiaXIE;
• 机 构: Department of Physiology, Medical College of Qingdao University, Qingdao 266071, China
• 出 处: 中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
• 发表日期: 2007年期

　　Divalent metal transporter 1 (DMT 1) is responsible for ferrous-ion transport.The over expression of DMT 1 in the brain has been suggested to be involved in iron accumulation in some neurodegenerative disorders.The aim of the present study is to construct two forms of DMT1 (with IRE and without IRE) over expression model to investigate the relationship between increased DMT1 expression and iron accumulation in MES23.5 cells,hence illustrating over expressed DMT1 resulted cell dysfunction.RT-PCR and western blot confirmed the increased DMT1 expression in AdDMT1+IRE and AdDMT1-IRE infected MES23.5 cells.