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Objective To investigate the association between expression of miRNA and efficacy of neoadjuvant chemotherapy of patients with breast cancer.Method Patients with at least four cycles of systemic neoadjuvant chemotherapy treatment were enrolled.pCR(pathologic complete response)and non-Pcr(non pathologic complete response)cohorts were included and archival needle aspiration tumor samples were obtained.miRNA expression profile was evaluated in the cohorts by the miRNA TaqMan Low-Density Arrays (TLDA).Target Gene Analysis was done for predicting target genes of candidate miRNAs and those genes were further analyzed for different signaling pathways by MAS 3.0 Cell viability assay, and transfction were performed for specific miRNAs in breast cancer cell lines.