【摘 要】
：
Acetylcholine is an important neurotransmitter in the brain andthe habenula-interpeduncular projection is a prominent cholinergic pathway.To study the physiological properties of cholinergic transmiss
【机 构】
：
College of Life Sciences, Beijing Normal University, Beijing 100875, China;National Institute of Bio
【出 处】
：
中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
Acetylcholine is an important neurotransmitter in the brain andthe habenula-interpeduncular projection is a prominent cholinergic pathway.To study the physiological properties of cholinergic transmission in the interpeduncular nucleus (IPN), we used a transgenic mouse line in which the light-gated cation channel channelrhodopsin-2 is selectively expressed in cholinergic neurons.Cholinergic axonal terminals were activated by light pulses and postsynaptic responses were recorded from IPN neurons.Surprisingly, brief photostimulation produces fast excitatory postsynaptic currents that were mediated by AMPA-type glutamate receptors, suggesting glutamate release at the synaptic sites.By contrast, tetanic photostimulation generates slow inward currents that are largely mediated by nicotinic acetylcholine receptors, suggesting volume transmission of acetylcholine.Finally, vesicular transporters for glutamate and acetylcholine are co-expressed on the same axonal terminals in the IPN.These results strongly suggest that adult brain "cholinergic" neurons can co-release glutamate and acetylcholine, but these two neurotransmitters activate postsynaptic neurons via different transmission modes.
其他文献
The molecular mechanisms underlying the behavioral effects of glucocorticoids are poorly understood.We report here that hippocampal neuronal nitric oxide synthase (nNOS) is a crucial mediator.Chronic
Voltage-gated sodium channel α-subunit type Ⅲ (Nav1.3) is mainly expressed in the central nervous system and is associated with neurological disorders.The expression of mouse Scn3a product (Nav1.3) ma
Introduction In noisy social gatherings, listeners perceptually integrate sounds originating from one persons voice (e.g., fundamental frequency (f0) and harmonics) at a particular location and segreg
Objective Autophagy is a process which can be activated during various stress conditions.Our previous study reported that oxidized low-density lipoprotein (ox-LDL) can activate autophagy in human umbi
The treatment of bone cancer pain remains a clinically challenging problem.Here, we report that A-type K+ channels in primary sensory neurons are involved in the neuropathy of rat bone cancer pain and
Objective The purpose of this study is to analyze the efficiency of neuro-mechanical compensation for kinematic variability during postural control.Signal dependent noise (SDN) in natural neuromuscula
Olfactory ensheathing cells (OECs) migrate from the olfactory epithelium towards the olfactory bulb during development.However, the guidance mechanism for OEC migration remains a mystery.Here we show
A negative event-related potential (ERP) component, known as N170, can be readily recorded over the posterior left brain region when skilled readers are presented with visual words.This left-lateraliz
Sigma receptors (σRs), which are classified into three subtypes (σR1, σR2, σR3), are widely distributed in the central nervous system.Our previous studies showed that σR1 was expressed in most of rat
Oscillatory firing is an important process in neural system for presenting, integrating and synchronizing neuronal signals.Though most pyramidal neurons are able to oscillate at a broad band, only a f